Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Alcian Blue 8GX
作者： Yuan Fang LI , Cheng Zhi HUANG , Xin Hua HUANG , Ming LI
关键词:
摘要: A protein assay with the determination sensitivity at nanogram levels has been developed based on measurement of enhanced resonance light scattering (RLS). In a neutral aqueous medium, interactions ABGX different proteins were found to result in strongly RLS signals. With signals 398.0 nm, proteins, including bovine serum albumin (BSA), human (HAS), pepsin (Pep) and cellulase (Cel), can be determined limit detection below 30 ng/ml. The results determinations for artificial samples agreement desired values, those identical obtained according Bradford method using CBB G-250.
sci-hub.se 参考文章(13)
Y WEI, K LI, S TONG, The interaction of Bromophenol Blue with proteins in acidic solution. Talanta. ,vol. 43, pp. 1- 10 ,(1996) , 10.1016/0039-9140(95)01683-X
Na Li, Ke-An Li, Shen-Yang Tong, Fluorometric Determination for Micro Amounts of Albumin and Globulin Fractions without Separation by Using α,β,γ,δ-Tetra(4′-carboxyphenyl)porphin Analytical Biochemistry. ,vol. 233, pp. 151- 155 ,(1996) , 10.1006/ABIO.1996.0022
T Rosenquist, D Stewart, Measurement of tissue disulfide groups by Alcian blue 8GX and atomic absorption spectrophotometry. Journal of Histochemistry and Cytochemistry. ,vol. 30, pp. 1311- 1312 ,(1982) , 10.1177/30.12.6185563
Jun-Xian Zhang, Peter J. Halling, pH and buffering in the bicinchoninic acid (4,4′-dicarboxy-2,2′-biquinoline) protein assay Analytical Biochemistry. ,vol. 188, pp. 9- 10 ,(1990) , 10.1016/0003-2697(90)90520-J
Cheng Zhi Huang, Ke An Li, Shen Yang Tong, Determination of nanograms of nucleic acids by their enhancement effect on the resonance light scattering of the cobalt(II)/4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex. Analytical Chemistry. ,vol. 69, pp. 514- 520 ,(1997) , 10.1021/AC960660F
R. Pasternack, P. Collings, Resonance light scattering : a new technique for studying chromophore aggregation Science. ,vol. 269, pp. 935- 939 ,(1995) , 10.1126/SCIENCE.7638615
Elena V. Yazynina, Anatoliy V. Zherdev, Boris B. Dzantiev, Vladimir A. Izumrudov, Shirley J. Gee, Bruce D. Hammock, Immunoassay techniques for detection of the herbicide simazine based on use of oppositely charged water-soluble polyelectrolytes. Analytical Chemistry. ,vol. 71, pp. 3538- 3543 ,(1999) , 10.1021/AC990072C
Robert F. Pasternack, Carlos Bustamante, Peter J. Collings, Antonino Giannetto, Esther J. Gibbs, Porphyrin Assemblies On DNA As Studied By A Resonance Light-Scattering Technique Journal of the American Chemical Society. ,vol. 115, pp. 5393- 5399 ,(1993) , 10.1021/JA00066A006
Yong-ju Wei, Ke-an Li, Shen-yang Tong, A linear regression method for the study of the Coomassie brilliant blue protein assay. Talanta. ,vol. 44, pp. 923- 930 ,(1997) , 10.1016/S0039-9140(96)02140-6
George A. Miller, Fluctuation theory of the resonance enhancement of Rayleigh scattering in absorbing media The Journal of Physical Chemistry. ,vol. 82, pp. 616- 618 ,(1978) , 10.1021/J100494A023