Methylcobalamin methylation of chloroplatinate: Bound chloride, valence state, and relative mutagenicity
作者： Robert T. Taylor , James A. Happe , Rebekah Wu
DOI: 10.1080/10934527809374849
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摘要: Abstract A methyl‐Pt compound formed by incubating K2PtCl6 with methylcobalamin (MeB‐12) is further characterized respect to its chloride content, Pt oxidation state, and electro‐phoretic mobility. The complexed has been determined from nuclear magnetic resonance (NMR) spectra of the Cl displaced competing thiocyanate (SCN ) anions. Release 3 chlorides per combined a Me/Pt ratio 1.0 indicates metal coordination number 4. This in agreement X‐ray photoelectron (ESCA) which show that majority Me‐Pt product contains +2 valence state. Based on this data anionic nature at pHs 2.5–7.5 it 2‐is presently represented as MePtCl3. In comparison closely related complexes K2PtCl4 K2PtCl6, MePtCl3 ‐2 slightly less cytotoxic an auxotrdphic Chinese hamster ovary cell line (CHO AUXB1). All three these chloro‐Pt compounds concentrations 15–70 μ? induce 6–10 fold increase frequency o...
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