cGMP modulates stem cells differentiation to neurons in brain in vivo.

摘要: Abstract During brain development neural stem cells may differentiate to neurons or other cell types. The aim of this work was assess the role cGMP (cyclic GMP) in modulation differentiation non-neuronal cells. fetuses reduced 46% controls by treating pregnant rats with nitroarginine-methylester ( l -NAME) and restored co-treatment sildenafil.Reducing during leads increased number prefrontal cortex originated from proliferating on gestational day 14 715±14/mm 2 control 440±29/mm (61% control) treated -NAME. In exposed -NAME plus sildenafil, completely normalized, reaching 683±11 neurons/mm . sildenafil alone labelled bromodeoxyuridine (BrdU) NeuN 841±16/mm 48% neurons, but only 24% This corrected 40% neurons. Similar results were obtained for all developmental period. Treatment did not reduce total BrdU, further supporting that reduces selectively hippocampus. although effect milder than cortex. These support modulates fate vivo suggest high levels promote its while