Large increase of rotavirus diarrhoea in the hospital setting associated with emergence of G12 genotype in a highly vaccinated population in Nicaragua

摘要: Rotaviruses (RVs) are a major cause of severe diarrhoea in young children. Nicaragua introduced routine immunization with the pentavalent RV vaccine (RV5) 2006, which greatly reduced incidence diarrhoea. A remaining concern has been possible emergence new strains to vaccination less effect. In this study, 837 children hospital settings were investigated for between May 2011 and July 2013. RVs subsequently typed by multiplex PCR and/or sequencing. Fecal anti-RV IgA titres subset RV-infected (n = 137) noninfected 52) determined an in-house enzyme-linked immunosorbent assay. The detection rate was 8% 2011, followed sharp increase 29% 2012 19% This associated predominance genotype G12 RV, from 0% 66% 82% 2013, infecting 1 month 10 years age. Two sequenced showed Wa-like genome G12-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, similar globally emerging strains. analysis that most G12-infected had contact either or wild strains, but such antibodies did not prevent symptomatic infection. marked evident setting nationwide population high RV5 coverage.