Detection of the emerging rotavirus G12P[8] genotype at high frequency in brazil in 2014: Successive replacement of predominant strains after vaccine introduction.

摘要: The continuum characterization of rotavirus (RVA) genotypes is essential to understand how vaccine introduction could impact virus epidemiology. In the present study, an unexpected rapid changing pattern RVA distribution in Brazilian population during three followed seasons described. From January/2012 December/2014, a total 3441 fecal specimens were collected from collaborating centers across Southern, Southeastern and Midwest Brazil. All screened for using ELISA, genotyped by RT-PCR. Differences proportions tested Chi-Squares. A p-value less than 0.05 was considered statistically significant. detected 19.7% (677/3441). Among positive cases (n=677), 652 (96.3%) samples successfully amplified G3P[8] remained prevalent 2012 (37.6%, 69/185) 2013 (40.1%, 74/186) (χ(2)=0.107, p=0.743), but declined markedly 2014 (3.5%, 10/281) (χ(2)=71.770, p=0.000). G12P[8] second highest strain (22.7%, 42/185), decrease rapidly (2.7%, 5/186) (χ(2)=26.224, p=0.000) re-emerged as predominant genotype (86.6%, 243/281) (χ(2)=118.299, July/2014, single all regions studied. sudden emergence, spread predominance Brazil, raised hypothesis possible G12 outbreak being progress. Nationally, long term decline gastroenteritis hospitalization observed country after confirmed. Nevertheless, sharp increase diarrhea prevalence Southern consistent with what appears be G12P[8]. Continued surveillance needed verify effectiveness RotarixTM Brazil together potential emergence unusual genotypes.