A one-step real-time RT-PCR helps to identify mixed rotavirus infections in Mexico
作者： Yazmin Anaya-Molina , Sergio Isaac De La Cruz Hernández , Atenea Estela Andrés-Dionicio , Heidi Lizbeth Terán-Vega , Héctor Méndez-Pérez
DOI: 10.1016/J.DIAGMICROBIO.2018.06.023
关键词:
摘要: Abstract Rotaviruses continue being the most important pathogens responsible of diarrhea in young children worldwide. Seminested reverse transcription polymerase chain reaction (RT-PCR) is used to determine rotavirus genotype; however, this technique employs multistep procedures. The real-time RT-PCR a fast and reliable tool that can be as genotyping tool, especially outbreaks. In study, we tested identify genotype using panel 252 samples from patients with diarrheal disease caused by G9P[4] G12P[8] genotypes, which were identified emerging rotaviruses 2 outbreaks Chiapas, Mexico. Our results show assay detected these rotaviruses, it allowed us mixed infections, G/P combinations, viral abundance some seminested could not them. Therefore, molecular great support during
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