Mapping of Digitaria streak virus transcripts reveals different RNA species from the same transcription unit.

作者: G. P. Accotto , J. Donson , P. M. Mullineaux

DOI: 10.1002/J.1460-2075.1989.TB03470.X

关键词:

摘要: All, except 19 [corrected] bp, of the Digitaria streak virus (DSV) genome is transcribed. Two RNA transcripts (1+ and 2+) are encoded by virion DNA strand up to five (1- 5-) complementary [corrected]. Detailed mapping these RNAs has revealed evidence for splicing in one species (RNA 4-), which together with its more abundant unspliced counterpart 2-) could synthesize both a 30.5 41 kd polypeptide from same transcription unit. This extensive overlapping spliced indicate that initiation temporally regulated. At least transcript 1-) may have non-translational role. Transcription DSV shows similarities some animal viruses, particularly papovaviruses.

参考文章(3)
Crispin J. Woolston, Richard Barker, Helen Gunn, Margaret I. Boulton, Philip M. Mullineaux, Agroinfection and nucleotide sequence of cloned wheat dwarf virus DNA Plant Molecular Biology. ,vol. 11, pp. 35- 43 ,(1988) , 10.1007/BF00016012
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)
J. W. DAVIES, J. STANLEY, J. DONSON, P. M. MULLINEAUX, M. I. BOULTON, Structure and replication of geminivirus genomes. Journal of Cell Science. ,vol. 1987, pp. 95- 107 ,(1987) , 10.1242/JCS.1987.SUPPLEMENT_7.7