Sterol Biosynthesis in Yeast

摘要: Anaerobically and aerobically grown yeast contains 3-hydroxy-3-methylglutaryl-CoA reductase, which is located in the mitochondrial fraction of cell. has a low specific activity reductase sterol content. Aeration this buffer, without growth, results an increase enzyme, paralleled by This induction oscillatory profile with anaerobically for 24 h linear pattern cells 72 h. With latter type yeast, glucose necessary induction, whereas other occurs glucose. By anaerobic incubation buffer h, can be transformed into one. The extent strictly dependent on concentration present. Sterols whole cells, but they do not fraction. strongly inhibited cycloheximide, affected chloramphenicol. enzyme closely connected metabolism cell; fructose, mannose, ethanol also induce reductase.