Genome‐wide screening reveals that miR‐195 targets the TNF‐α/NF‐κB pathway by down‐regulating IκB kinase alpha and TAB3 in hepatocellular carcinoma

摘要: Nuclear factor kappa B (NF-κB) is an important linking inflammation and tumorigenesis. In this study we experimentally demonstrated through a high-throughput luciferase reporter screen that NF-κB signaling can be directly targeted by nearly 29 microRNAs (miRNAs). Many of these miRNAs target nodes binding to their 3′ untranslated region (UTR). miR-195, member the miR-15 family, frequently down-regulated in gastrointestinal cancers, especially hepatocellular carcinoma (HCC). The expression level miR-195 inversely correlated with HCC tumor size. We further show suppresses cancer cell proliferation migration vitro reduces tumorigenicity metastasis vivo. Additionally, may exert its suppressive function decreasing multiple downstream effectors way direct targeting IKKα TAB3. Conclusion: Multiple are involved pathway plays inhibitory roles progression potential therapeutic target. (Hepatology 2013;58:654–666)