MicroRNA-92b promotes tumor growth and activation of NF-κB signaling via regulation of NLK in oral squamous cell carcinoma

摘要: miR-92b has been reported to be dysregulated in many types of human cancers. However, the role oral squamous cell carcinoma (OSCC) is unknown. The aim present study was investigate function and mechanism OSCC. Using quantitative reverse‑transcription PCR (qRT-PCR), we found that level primary tumors (n=85) significantly elevated compared with adjacent normal tissues (p<0.001). A high associated a large tumor size (p=0.005), advanced stage (p<0.001) poorer prognosis (p=0.04). Functionally, shown not only promote proliferation OSCC cells MTT colony formation xenograft assays, but also inhibit apoptosis flow cytometric assay. In western blotting luciferase assay, NLK identified as direct functional target miR-92b. We involved miR-92b-induced proliferation, its protein obviously downregulated miR-92b-overexpressing tumors. Finally, reporter assay fluorescent immunostaining revealed activated NF-κB signaling pathway, which may responsible for effects on proliferation. Taken together, our results indicate upregulation accelerates growth novel miRNA‑mediated activation