Hesperetin post-treatment prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating PI3K/Akt signaling pathway
作者： Shangfei He , Xianbao Wang , Yongkang Zhong , Lu Tang , Ya Zhang
DOI: 10.1016/J.BIOPHA.2017.05.003
关键词:
摘要: Hesperidin (HES), a citrus fruit extract, has beneficial effects on various ischemia/reperfusion (I/R) models. Here, we investigated the possible positive effect of hesperetin (HPT), an active metabolite HES, and identified potential molecular mechanisms involved in cardiomyocytes H/R-induced injury. To construct cardiomyocyte model hypoxia/reoxygenation (H/R) injury, cultured neonatal rat were subjected to 3h hypoxia followed by reoxygenation. Cell viability apoptosis detected. The levels Apoptosis-related proteins PI3K/Akt detected western blot. Our results showed that HPT post-treatment significantly inhibited elevating expression Bcl-2, decreasing Bax cleaved caspase-3, diminished apoptotic ratio. Mechanism studies demonstrated up-regulated p-PI3K, p-Akt. Co-treatment with PI3K/Akt-specific inhibitor LY294002 blocked HPT-induced cardioprotective effects. Taken together, these data suggested prevented from H/R injury vitro most likely through activation signaling pathway.
sci-hub.se 参考文章(33)
Akito Shimouchi, Harumasa Yokota, Shinji Ono, Chiemi Matsumoto, Toshihiro Tamai, Hiroko Takumi, Subbadra P Narayanan, Shoji Kimura, Hiroya Kobayashi, Ruth B Caldwell, Taiji Nagaoka, Akitoshi Yoshida, None, Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury Japanese Journal of Ophthalmology. ,vol. 60, pp. 51- 61 ,(2016) , 10.1007/S10384-015-0415-Z
Yi Yu, Ronald E. Savage, Sudharshan Eathiraj, Justin Meade, Michael J. Wick, Terence Hall, Giovanni Abbadessa, Brian Schwartz, Targeting AKT1-E17K and the PI3K/AKT Pathway with an Allosteric AKT Inhibitor, ARQ 092 PLOS ONE. ,vol. 10, ,(2015) , 10.1371/JOURNAL.PONE.0140479
Shulin Wang, Yunpeng Li, Xudong Song, Xianbao Wang, Cong Zhao, Aihua Chen, Pingzhen Yang, Febuxostat pretreatment attenuates myocardial ischemia/reperfusion injury via mitochondrial apoptosis Journal of Translational Medicine. ,vol. 13, pp. 209- 209 ,(2015) , 10.1186/S12967-015-0578-X
Tao Luo, Baihe Chen, Zonglei Zhao, Nvqin He, Zhi Zeng, Bing Wu, Yasushi Fukushima, Meng Dai, Qiaobing Huang, Dingli Xu, Jianping Bin, Masafumi Kitakaze, Yulin Liao, Histamine H2 receptor activation exacerbates myocardial ischemia/reperfusion injury by disturbing mitochondrial and endothelial function Basic Research in Cardiology. ,vol. 108, pp. 342- 342 ,(2013) , 10.1007/S00395-013-0342-4
Wei-Li Qiao, Differential expression of Bcl-2 and Bax during gastric ischemia-reperfusion of rats World Journal of Gastroenterology. ,vol. 17, pp. 1718- 1724 ,(2011) , 10.3748/WJG.V17.I13.1718
Krishnendu Sinha, Joydeep Das, Pabitra Bikash Pal, Parames C. Sil, Oxidative stress: the mitochondria-dependent and mitochondria-independent pathways of apoptosis Archives of Toxicology. ,vol. 87, pp. 1157- 1180 ,(2013) , 10.1007/S00204-013-1034-4
Ayse Nur Cakir Gungor, Meryem Gencer, Turan Karaca, Servet Hacivelioglu, Ahmet Uysal, Fatma Korkmaz, Selim Demirtas, Emine Cosar, None, The effect of hesperetin on ischemia–reperfusion injury in rat ovary Archives of Gynecology and Obstetrics. ,vol. 290, pp. 763- 769 ,(2014) , 10.1007/S00404-014-3267-8
Ali Roohbakhsh, Hamideh Parhiz, Fatemeh Soltani, Ramin Rezaee, Mehrdad Iranshahi, Molecular mechanisms behind the biological effects of hesperidin and hesperetin for the prevention of cancer and cardiovascular diseases. Life Sciences. ,vol. 124, pp. 64- 74 ,(2015) , 10.1016/J.LFS.2014.12.030
QINGQING WU, HENG ZHOU, ZHOUYAN BIAN, QIZHU TANG, Hesperetin attenuates mitochondria-dependent apoptosis in lipopolysaccharide-induced H9C2 cardiomyocytes Molecular Medicine Reports. ,vol. 9, pp. 1941- 1946 ,(2014) , 10.3892/MMR.2014.2002
L. C. Cantley, The Phosphoinositide 3-Kinase Pathway Science. ,vol. 296, pp. 1655- 1657 ,(2002) , 10.1126/SCIENCE.296.5573.1655