Equilibrium theory of ion exchange chromatography with variable solution normality and steric hindrance
作者： M. Fechtner , A. Kienle
DOI: 10.1016/J.CES.2019.01.040
关键词:
摘要: Abstract The paper extends equilibrium theory of ion exchange chromatography to account for variable solution normality and steric hindrance. Both effects are crucial many separations including the separation proteins. Analytical solutions given a full chromatographic cycle consisting loading an empty bed equilibrated at different salt concentrations followed by regeneration step. Special emphasis is on selectivity reversals. It shown that additional reversals may occur due change in normality. Results illustrated step relation with constant and/or mass action without hindrance discussed. Theoretical findings validated comparison numerical simulation.
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