Changes of Red Blood Cell Surface Markers in a Blood Doping Model of Neocytolysis

摘要: Background: Neocytolysis, the selective hemolysis of young circulating red blood cells (RBCs), contributes to physiologic control cell mass and pathophysiologic phenomena such as anemia renal disease, after spaceflight, doping by athletes. Progress in understanding process is hampered lack established markers distinguish from older RBC. Methods: Twelve potentially informative RBC surface were assayed flow cytometry normal samples, 4 preferentially expressed To create a model neocytolysis, 3 volunteers had recombinant human erythropoietin (rhEpo) administered until mild erythrocytosis occurred, then studied upon rhEpo withdrawal. Results: Neocytolysis ensued that most evident rapid rise serum ferritin iron was transferred back stores. Five additional monitored during administration. Three subjects with marginal baseline stores blunted response rhEpo, no significant change marker expression. Two adequate developed followed neocytolysis. Decreased expression CD44 (homing-associated adhesion molecule) CD71 (transferrin receptor) seemed correlate best neocytolysis; CD35 (complement less so. Of note, further studies are needed determine if these changes causative destruction. Conclusion: This study begins establish differentiating old RBC, basis for better definition process. Although our preliminary, results support possibility could be useful detect because neocytolysis should predictably occur athletes who surreptitiously dope.