Density fractionation of erythrocytes by Percoll/hypaque results in only a slight enrichment for aged cells.

摘要: Abstract Density fractionation has served for many years as a standard procedure the isolation of aged erythrocyte populations; however, quantitative evaluation technique not been available. This report describes such an analysis. Rabbits were infused intravenously with N-hydroxysuccinimido biotin to biotinylate greater than 90% all circulating erythrocytes; enumeration these biotinylated cells was possible by monitoring their ability bind avidin-coated microspheres. The erythrocytes shown have normal in vivo survival. As result senescence process, only red would membrane-bound 50 days after biotinylation. At this time, rabbit fractionated over Percoll/hypaque determine density enrich aged, cells. resulted average enrichment 2–3-fold above that present random population.