The kinetic determination of clinically significant enzymes in an automated flow-injection system with fluorescence detection
作者： M Masoom , PJ Worsfold , None
DOI: 10.1016/S0003-2670(00)84466-9
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摘要: Abstract An automated flow-injection manifold is described for the kinetic determination of enzyme activities by a stopped-flow procedure with fluorescence detection. The linear calibration range alkaline phosphatase 0/2-250 U l−1 precision 2%; sample thoughput 35/2-40 h−1. Linear responses were also obtained lipase (0/2-100 l−1), acethylcholinesterase (0/2-500 l−1) and chymotrypsin (0/2-200 l−1). advantages this approach to plasma include sensitivity small reagent volumes needed.
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