Expression and prognostic relevance of activated extracellular-regulated kinases (ERK1/2) in breast cancer.

摘要: Extracellular-regulated kinases (ERK1, ERK2) play important roles in the malignant behaviour of breast cancer cells vitro. In our present study, 148 clinical samples (120 cases with follow-up data) were studied for expression ERK1, ERK2 and their phosphorylated forms p-ERK1 p-ERK2 by immunoblotting, p-ERK1/2 corresponding paraffin sections was analysed immunohistochemistry. The results correlated established histological prognostic parameters, data seven cell-cycle regulatory proteins as well MMP1, MMP9, PAI-1 AP-1 transcription factors, which had been before. High determined immunoblots significantly a low frequency recurrences infrequent fatal outcome (P=0.007 0.008) an independent indicator long relapse-free overall survival multivariate analysis. By immunohistochemistry, strong p-ERK staining tumour associated early stages (P=0.020), negative nodal status (P=0.003) recurrence-free (P=0.017). contrast, unphosphorylated ERK1 not except positive correlation oestrogen receptor status. Comparison formerly cell-cycle- invasion-associated corroborates conclusion that activation is enhanced proliferation invasion mammary carcinomas.