Catabolism of camphor in tissue cultures and leaf disks of common sage (Salvia officinalis)

摘要: Abstract (+)-Camphor constitutes nearly 30% of the monoterpenes accumulated in leaves common sage (Salvia officinalis), and as plant approaches maturity content this monoterpene ketone decreases by roughly half. Although ability to catabolize camphor has been demonstrated previously leaf disks, tissue cultures proved be a more suitable system for examining responsible degradative pathway. Cell suspension were shown convert (+)-[3-3H2]camphor, sequence, 6-hydroxycamphor, 6-oxocamphor, α-campholonic acid, 2-hydroxy-α-campholonic each intermediate pathway was identified chromatographic spectroscopic means. This oxidative ring opening sequence resembles degradation soil diphtheroid, Mycobacterium rhodochrous, that ultimately leads isoketocamphoric last defined metabolite contains all 10 carbons original bicyclic nucleus. Studies with both cell disks also catabolism via 1,2-campholide, described, minor The first step metabolism cell-free extracts cultured cells, several lines evidence indicated microsomal (+)-camphor-6-exo-hydroxylase is cytochrome P450-dependent monooxygenase.