Independent cadherin-catenin and Bazooka clusters interact to assemble adherens junctions.

摘要: Proper epithelial structure requires adherens junction (AJ) assembly. In the early Drosophila embryo, AJ assembly depends on Bazooka (Baz; PAR-3), but it is unclear how Baz affects and what precursors are involved. To understand this process at molecular level, we counted number of core proteins an average spot (SAJ) determined their dynamics with fluorescence recovery after photobleaching experiments. These data reveal that SAJs subdivided into clusters cadherin–catenin independent protein numbers dynamics. This independence suggests precursory might form before SAJ We identify forming between apical microvilli. Further analyses show they independently functions in repositioning them to apicolateral sites for full Our implicate cell protrusions initial clustering melanogaster embryo. Then, appear engage assemble SAJs.