Permeability of Mouse Oocytes and Embryos at Various Developmental Stages to Five Cryoprotectants

摘要: To assess the permeability of mouse oocytes and embryos, matured embryos at various stages development were placed in five cryoprotectant solutions 25 C for min. From cross-sectional areas oocytes/embryos, relative change volume was analyzed. In oocytes, shrinkage least extensive recovery quickest propylene glycol solution, showing that permeates most rapidly. Dimethyl sulfoxide, acetamide, ethylene permeated slightly more slowly than glycol. The glycerol shrunk extensively then expanded marginally, indicating slow permeation. changes 1-cell 2-cell similar to those little permeability. 8-cell recovered much faster earlier especially acetamide. morulae, glycol; glycol, extent small fast, an extremely rapid Although oocytes/embryos generally increased as embryo proceeded, degree increase varied greatly among cryoprotectants. Interestingly, virtually unaffected by stage development. Such information will be valuable determining a suitable protocol cryopreservation different