Effect of bovine oviduct epithelial cell apical plasma membranes on sperm function assessed by a novel flow cytometric approach.

摘要: In the bovine, as in many mammalian species, sperm are temporarily stored oviduct before fertilization by binding to epithelial cell apical plasma membranes. As is able maintain motility and viability of modulate capacitation, we propose that proteins present on membrane cells contribute these effects. To verify this hypothesis, frozen-thawed was determined after incubation for 6 h with purified membranes from fresh or cultured bovine mammary gland a control. Analysis intracellular calcium levels performed flow cytometry incubated using Indo-1 assess effect concentration. The coculture maintained initial (65% 84%, respectively). This significantly different control without (23%), boiled (21%). Apical diminished percentage reached lethal concentration over 4-h period (18.7%) compared (53.8%) lower viable sperm. These results show contains anchored proteinic factors maintaining possibly modulating capacitation