Affinity-based fluorogenic labeling of ATP-binding proteins with sequential photoactivatable cross-linkers.
作者： Takenori Tomohiro , Hirotsugu Inoguchi , Souta Masuda , Yasumaru Hatanaka
DOI: 10.1016/J.BMCL.2013.08.041
关键词:
摘要: A specific illumination approach has been developed for identification of adenosine triphosphate (ATP)-binding proteins. This strategy utilizes a tandem photoactivatable unit that consists diazirine group as carbene precursor and an o-hydroxycinnamate moiety coumarin precursor. The photolysis induces cross-link on target proteins is followed by photoactivation generation with concomitant release the pre-installed affinity ligand. ATP, installed this cross-linker at γ-position, successfully transferred onto ATP-binding using only UV-irradiation.
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