Development and characterization of monoclonal antibody against human IL-37b.
作者： Yu-Chi Gao , Yan Jia , De-Qian Xiao , Xin Wang , You-Chao Dai
DOI: 10.1007/S10616-016-0052-5
关键词:
摘要: IL-37 has been described as a natural inhibitor of immune responses. Monoclonal antibody (mAb) against human IL-37b with high affinity and specificity can serve molecular probe to detect study functions, mechanisms related signal pathways in inflammatory diseases. However, there are very few such mAbs commercially available so far. In the current study, monoclonal antibodies were developed by fusing splenocytes from immunized mouse SP2/0 myeloma cells polyethylene glycol. Then screened prokaryotic expressed protein eukaryotic subsequently. Western blot flow cytometry analysis revealed that selected mAb clons able recognize specificity. And more importantly, fluorescently labeled be directly used in flow immunohistochemistry. conclusion, new IL-37b, which applicable
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