Improvement of lipid and phospholipid recoveries from sardine (Sardina pilchardus) viscera using industrial proteases
作者： J. Dumay , C. Donnay-Moreno , G. Barnathan , P. Jaouen , J.P. Bergé
DOI: 10.1016/J.PROCBIO.2006.04.005
关键词:
摘要: Enzymatic hydrolysis of sardine viscera by three broad spectrum proteases was investigated using the pHstat method (24 h, pH 8, 50 °C). After hydrolysis, fractions (sludge, aqueous phase and oily phase) were collected. For each fraction, lipids phospholipids quantified molecular weights peptides determined. Under these conditions, degree (DH) varied from 1.9% (Flavourzyme), 3.1% (Protamex) to 3.3% (Alcalase). Dry matter distribution indicated that all performed increased solubilisation proteins: protein recovery for ranged 57.4% 61.2%. Furthermore, total lipid content in liquid parts (oily phases) (at least 85% raw material are which may improve commercial applications. In addition, richer than those extracted classical chemical extraction, especially after Alcalase hydrolysis.
elsevier.com
core.ac.uk 
sci-hub.se 参考文章(37)
Karl Y. Hostetler, Sunan Chaidhawangul, James R. Beadle, Germaine Bergeron–Lynn, Lingyun Cheng, William R. Freeman, Kelly A. Soules, Michael F. Gardner, Arthur J. Mueller, Gregory M. Severson, Kelly S. Keefe, Intravitreal toxicology and duration of efficacy of a novel antiviral lipid prodrug of ganciclovir in liposome formulation. Investigative Ophthalmology & Visual Science. ,vol. 41, pp. 1523- 1532 ,(2000)
J Dumay, C Barthomeuf, JP Bergé, How Enzymes May Be Helpful for Upgrading Fish By-Products: Enhancement of Fat Extraction Journal of Aquatic Food Product Technology. ,vol. 13, pp. 69- 84 ,(2004) , 10.1300/J030V13N02_07
Michael Schneider, Phospholipids for functional food European Journal of Lipid Science and Technology. ,vol. 103, pp. 98- 101 ,(2001) , 10.1002/1438-9312(200102)103:2<98::AID-EJLT98>3.0.CO;2-G
K Y Hostetler, D D Richman, C N Sridhar, P L Felgner, J Felgner, J Ricci, M F Gardner, D W Selleseth, M N Ellis, Phosphatidylazidothymidine and phosphatidyl-ddC: assessment of uptake in mouse lymphoid tissues and antiviral activities in human immunodeficiency virus-infected cells and in Rauscher leukemia virus-infected mice. Antimicrobial Agents and Chemotherapy. ,vol. 38, pp. 2792- 2797 ,(1994) , 10.1128/AAC.38.12.2792
Masahiro Murakami, Kentaro Makabe, Shigeru Okada, Katsumi Yamaguchi, Shoji Konosu, Screening of biologically active compounds in microalgae Nippon Suisan Gakkaishi. ,vol. 54, pp. 1035- 1039 ,(1988) , 10.2331/SUISAN.54.1035
John Charles Marshall Stewart, Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry. ,vol. 104, pp. 10- 14 ,(1980) , 10.1016/0003-2697(80)90269-9
Bjoern Liaset, E Lied, M Espe, Enzymatic hydrolysis of by‐products from the fish‐filleting industry; chemical characterisation and nutritional evaluation Journal of the Science of Food and Agriculture. ,vol. 80, pp. 581- 589 ,(2000) , 10.1002/(SICI)1097-0010(200004)80:5<581::AID-JSFA578>3.0.CO;2-I
F. Caponio, A. Lestingi, C. Summo, M. T. Bilancia, V. Laudadio, Chemical characteristics and lipid fraction quality of sardines (Sardina pilchardus W.): influence of sex and length Journal of Applied Ichthyology. ,vol. 20, pp. 530- 535 ,(2004) , 10.1111/J.1439-0426.2004.00611.X
Asbjørn Gildberg, Even Stenberg, A new process for advanced utilisation of shrimp waste Process Biochemistry. ,vol. 36, pp. 809- 812 ,(2001) , 10.1016/S0032-9592(00)00278-8
Kimiko Murakami-Murofushi, Susumu Kobayashi, Kenjiro Onimura, Miyoko Matsumoto, Masaki Shioda, Shorten Yoshida, Mami Shoji, Hiromu Murofushi, Selective inhibition of DNA polymerase-α family with chemically synthesized derivatives of PHYLPA, a unique Physarum lysophosphatidic acid Biochimica et Biophysica Acta. ,vol. 1258, pp. 57- 60 ,(1995) , 10.1016/0005-2760(95)00097-V