8-Oxoguanine incision activity is impaired in lung tissues of NSCLC patients with the polymorphism of OGG1 and XRCC1 genes.

摘要: Decreased repair of oxidative DNA damage is a risk factor for developing certain human malignancies. We have previously found that the capacity 8-oxo-7,8-dihydroguanine was lower in leukocytes NSCLC patients than controls. To explain these observations, we searched mutations and polymorphisms OGG1 gene among 88 79 One patient exhibited heterozygous mutation exon 1, which resulted Arg46Gln substitution. Normal lung tumor tissue carrying this showed markedly 8-oxoG incision activity mean all patients. The predominant polymorphism Ser326Cys. A significant difference observed frequencies variants between populations frequency Cys326 allele number Cys326Cys homozygotes higher In individuals with either Ser326Cys or genotype rate those both Ser326 alleles, leukocytes. Moreover, 8-oxodG level two alleles genotype. also screened XRCC1 gene. Only heterozygotes Arg194Trp, Arg280His Arg399Gln were controls, being significantly revealed their tissues, but not can conclude may an impact on efficiency humans His280 Gln399 influence tissues exposed to chronic oxidative/inflammatory stress. Higher partially impairment