Comparative study of influenza virus replication in MDCK cells and in primary cells derived from adenoids and airway epithelium

摘要: Although clinical trials with human subjects are essential for determination of safety, infectivity, and immunogenicity, it is desirable to know in advance the infectiousness potential candidate live attenuated influenza vaccine strains use. We compared replication kinetics wild-type viruses, including H1N1, H3N2, H9N2, B strains, Madin-Darby canine kidney (MDCK) cells, primary epithelial cells derived from adenoids, bronchial epithelium (NHBE cells). Our data showed that despite fact all tissue culture models lack a functional adaptive immune system, differentiated cultures exhibited greatest restriction viruses studied among three cell types tested best correlation their levels attenuation seen humans. In contrast, obtained MDCK were least predictive restricted viral able detect statistically significant difference between abilities U.S. (A/Ann Arbor/6/60) Russian (A/Leningrad/134/17/57) cold-adapted donor NHBE cultures. Since pandemic vaccines may potentially express hemagglutinin neuraminidase non-human virus, we assessed which could be used optimally evaluate infectivity cellular tropism different hosts. Among tested, most adequately reflected virus receptor specificities. considered use as screening step evaluating candidates.