Evolutionary and sequence-based relationships in bacterial AdoMet-dependent non-coding RNA methyltransferases

摘要: RNA post-transcriptional modification is an exciting field of research that has evidenced this editing process as a sophisticated epigenetic mechanism to fine tune the ribosome function and control gene expression. Although tRNA modifications seem be more relevant for cell physiology whole, some rRNA have also been seen play pivotal roles, essentially those located in central regions. methylation at nucleobases ribose moieties nucleotides appear frequently modulate its chemistry structure. methyltransferases comprise superfamily highly specialized enzymes accomplish wide variety modifications. These exhibit poor degree sequence similarity spite using common reaction cofactor modifying same substrate type. Relationships lineages extensively discussed, but no consensus reached. To shed light on topic, we performed amino acid codon-based analyses determine phylogenetic relationships molecular evolution. We found most Class I MTases are evolutionarily related protein cofactor/vitamin biosynthesis methyltransferases. Additionally, least nine explain diversity MTases. high content polar positively charged acid, which coincides with electrochemistry their substrates. After studying almost 12,000 bacterial genomes 2,000 patho-pangenomes, revealed evolution matches different rates synonymous non-synonymous substitutions along coding region. Consequently, selects against changes affecting structure conformation.