作者: Jennifer Lippincott‐Schwartz , George H. Patterson
DOI: 10.1016/S0091-679X(08)85003-0
关键词:
摘要: The discoveries, improvements, and alterations of fluorescent protein (FP) variants are having a profound impact on the ability investigators to observe quantify behavior proteins within cells organisms. Among most promising FPs photoactivatable (PA-FPs). Invisible at imaging wavelength until activated by irradiation different wavelength, PA-FPs allow controlled highlighting distinct molecular populations cell. This chapter introduces types discusses their use for monitoring movement, turnover, interactions, high-resolution localization.