Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR.
作者： Sérgio Monteiro de Almeida , Sônia Mara Raboni , Meri Bordignon Nogueira , Luine R. Renaud Vidal
DOI: 10.1590/0004-282X20160119
关键词:
摘要: The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). aim this study was to examine the influence red blood cells (RBC)s cerebrospinal fluid (CSF) as reverse transcription (RT-PCR) enteroviruses (EV). Forty-four CSF from patients showing characteristics viral meningitis were assessed EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection performed in-house nested PCR. Positivity RT-PCR higher without RBCs than RBCs: 13(26%) 36(9.2%), p = 0.001. In group positive RT-PCR, mean + SD RBC 37 ± 183 cell/mm3; negative results had 580 2,890 cell/mm3 (p 0.007). acceptable upper limit that could not 108 cells/mm3. have more erythrocytes.
sci-hub.se 参考文章(19)
Annette Spreer, Detection of Infectious Agents Springer, Cham. pp. 131- 142 ,(2015) , 10.1007/978-3-319-01225-4_11
Atsushi Akane, Kazuo Matsubara, Hiroaki Nakamura, Setsunori Takahashi, Kojiro Kimura, Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification Journal of Forensic Sciences. ,vol. 39, pp. 13607J- 372 ,(1994) , 10.1520/JFS13607J
Luine Rosele Renaud Vidal, Sérgio Monteiro de Almeida, Iara José de Messias-Reason, Meri Bordignon Nogueira, Maria do Carmo Debur, Luís Felipe Cavalli Pessa, Luciane Aparecida Pereira, Indianara Rotta, Gislene Reche de Almeida Takahashi, Clyete Santos da Silveira, Josianne Maria Reimann Araújo, Sonia Mara Raboni, Enterovirus and herpesviridae family as etiologic agents of lymphomonocytary meningitis, Southern Brazil Arquivos De Neuro-psiquiatria. ,vol. 69, pp. 475- 481 ,(2011) , 10.1590/S0004-282X2011000400013
I. Casas, F. Pozo, G. Trallero, J.M. Echevarr�a, A. Tenorio, Viral diagnosis of neurological infection by RT multiplex PCR: a search for entero- and herpesviruses in a prospective study. Journal of Medical Virology. ,vol. 57, pp. 145- 151 ,(1999) , 10.1002/(SICI)1096-9071(199902)57:2<145::AID-JMV10>3.0.CO;2-N
A S Ochert, A W Boulter, W Birnbaum, N W Johnson, C G Teo, Inhibitory effect of salivary fluids on PCR: potency and removal. Genome Research. ,vol. 3, pp. 365- 368 ,(1994) , 10.1101/GR.3.6.365
J Satsangi, DP Jewell, K Welsh, M Bunce, JI Bell, None, Effect of heparin on polymerase chain reaction The Lancet. ,vol. 343, pp. 1509- 1510 ,(1994) , 10.1016/S0140-6736(94)92622-0
Elisabeth Puchhammer-Stöckl, Elisabeth Presterl, Cornelia Croÿ, Stefan Aberle, Therese Popow-Kraupp, Michael Kundi, Hanns Hofmann, Ursula Wenninger, Irene Gödl, Screening for possible failure of herpes simplex virus PCR in cerebrospinal fluid for the diagnosis of herpes simplex encephalitis. Journal of Medical Virology. ,vol. 64, pp. 531- 536 ,(2001) , 10.1002/JMV.1082
I. Casas, A. Tenorio, J.M. Echevarria, P.E. Klapper, G.M. Cleator, Detection of enteroviral RNA and specific DNA of herpesviruses by multiplex genome amplification Journal of Virological Methods. ,vol. 66, pp. 39- 50 ,(1997) , 10.1016/S0166-0934(97)00035-9
I. Casas, L. Powell, P.E. Klapper, G.M. Cleator, New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay Journal of Virological Methods. ,vol. 53, pp. 25- 36 ,(1995) , 10.1016/0166-0934(94)00173-E
Anthony K Akobeng, Understanding diagnostic tests 3: receiver operating characteristic curves Acta Paediatrica. ,vol. 96, pp. 644- 647 ,(2007) , 10.1111/J.1651-2227.2006.00178.X