p53 Mutation Is Infrequent in Clear Cell Carcinoma of the Ovary

摘要: Abstract Objective. p53 gene alteration has been extensively studied in epithelial ovarian cancer. However, its occurrence clear cell carcinoma, an infrequent histologic subtype of cancer, is rarely reported. The aim this study to determine the status distinct type carcinoma. Methods. Paraffin blocks tumors from 38 patients with primary or recurrent carcinoma were for alteration. All these subjected immunohistochemical and molecular analysis. Two monoclonal antibodies (DO-7 PAb 1801) used staining. Genomic DNAs extracted paraffin subscribed a nested polymerase chain reaction/single-strand conformation polymorphism (PCR/SSCP) Tumors showing band shift on SSCP further prepared DNA sequencing site mutation. Results. Overexpression was observed only one stage III focal positive staining noted another five tumors. Of six immunohistochemistry, alterations four Three revealed missense point mutation: two exon 7 (TCT 227 → TTT GGC 245 AGC) 5 (CGC 156 CAC). Another tumor 12-bp deletion possible ways: it might involve last codons at 3′ end 4 (nucleotides 12,288–12,299) cross over splice junction between intron 12,290–12,301). former would result predicted protein product 389 amino acids whereas latter cause frameshift sequence truncated protein. Conclusion. Mutations appear be much less frequent than other types We suggest that may not play important role development