MicroRNA Expression Is Down-Regulated and Reorganized in Prefrontal Cortex of Depressed Suicide Subjects

摘要: BACKGROUND: Recent studies suggest that alterations in expression of genes, including those which regulate neural and structural plasticity, may be crucial the pathogenesis depression. MicroRNAs (miRNAs) are newly discovered regulators gene have recently been implicated a variety human diseases, neuropsychiatric diseases. METHODOLOGY/PRINCIPAL FINDINGS: The present study was undertaken to examine whether miRNA network is altered brain depressed suicide subjects. Expression miRNAs measured prefrontal cortex (Brodmann Area 9) antidepressant-free (n = 18) well-matched non-psychiatric control subjects 17) using multiplex RT-PCR plates. We found overall significantly globally down-regulated Using individual tests statistical significance, 21 were decreased at p 0.05 or better. Many encoded nearby chromosomal loci, shared motifs within 5'-seeds, putative mRNA targets, several In addition, set 29 miRNAs, whose not pairwise correlated normal controls, showed high degree co-regulation across individuals group. CONCLUSIONS/SIGNIFICANCE: findings show widespread changes likely participate major depression and/or suicide. Further needed identify lead mRNAs, either directly (by acting as targets) indirectly (e.g., by affecting transcription factors). Language: en