The role of low levels of fullerene C60 nanocrystals on enhanced learning and memory of rats through persistent CaMKII activation.

摘要: Engineered nanomaterials are known to exhibit diverse and sometimes unexpected biological effects. Fullerene nanoparticles have been reported specifically bind elicit persistent activation of hippocampal Ca(2+)/calmodulin-dependent protein kinase II (CaMKII), a multimeric intracellular serine/threonine central Ca(2+) signal transduction critical for synaptic plasticity, but the functional consequence that modulation is unknown. Here we show low doses fullerene C60 nanocrystals (Nano C60), delivered through intrahippocampal infusion without any obvious cytotoxicity in neuronal cells, enhance long-term potentiation (LTP) rats. Intraperitoneal injection 320 μg/kg Nano C60, once daily 10 days, also enhanced spatial memory rats addition an increase LTP. In parallel, both IH IP administration increased autonomous activity level threonine 286 (T286) autophosphorylation CaMKII, post-synaptic AMPA/NMDA ratio, triggered time-dependent ERK CREB. Our results reveal striking highly ability positively modulating learning memory, effect most likely manifested locking CaMKII active conformation, may significant implications potential therapeutic applications classic engineered nanomaterial.