Carboxylic Acid Fullerene (C60) Derivatives Attenuated Neuroinflammatory Responses by Modulating Mitochondrial Dynamics

摘要: Fullerene (C60) derivatives, a unique class of compounds with potent antioxidant properties, have been reported to exert wide variety biological activities including neuroprotective properties. Mitochondrial dynamics are an important constituent cellular quality control and function, imbalance the eventually leads mitochondria disruption cell dysfunctions. This study aimed assess effects carboxylic acid C60 derivatives (C60–COOH) on mitochondrial elucidate its associated mechanisms in lipopolysaccharide (LPS)-stimulated BV-2 microglial model. Using cell-based functional screening system labeled DsRed2-mito cells, we showed that LPS stimulation led excessive fission, increased localization dynamin-related protein 1 (Drp1), both which were markedly suppressed by C60–COOH pretreatment. LPS-induced reactive oxygen species (ROS) generation collapse membrane potential (ΔΨm) also significantly inhibited C60–COOH. Moreover, found pretreatment resulted attenuation LPS-mediated activation nuclear factor (NF)-κB mitogen-activated kinase (MAPK) signaling, as well production pro-inflammatory mediators. Taken together, these findings demonstrated may through regulating functions thus providing novel insights into properties derivatives.