Transforming growth factor beta 1-induced changes in cell migration, proliferation, and angiogenesis in the chicken chorioallantoic membrane.

摘要: Application of TGF beta 1 (10-100 ng) to the chicken chorioallantoic membrane (CAM) for 72 h resulted in a dose-dependent, gross angiogenic response. The vascular effects induced by were qualitatively different than those maximal doses basic FGF (bFGF) (500 ng). While formation large blood vessels h, bFGF primarily small vessels. Histologic analysis revealed that stimulated pleiotropic cellular responses CAM. Increases fibroblast and epithelial cell density area delivery observed as early 4 after treatment. By 8 these types also demonstrated altered morphology marked inhibition proliferation evidenced 3H-thymidine labeling. Thus, 1-stimulated accumulation was result chemotaxis from peripheral areas into delivery. Microscopic angiogenesis form capillary sprouts increased endothelial first became evident at 16 h. 24 cords appeared within mesenchyme CAM, extending towards point labeling growth due proliferation. Finally, perivascular mononuclear inflammation did not become until 48 treatment, its presence correlated spatially temporally with histological remodelling newly formed larger In summary, data suggest that, initiates sequence results inhibition, through migration, microvascular angiogenesis.