Adaptation of an Enzyme Immunoassay to Assess Urinary Cotinine in Nonsmokers Exposed to Tobacco Smoke

摘要: Nicotine and its metabolites (1), expired carbon monoxide, thiocyanates (2) are the most widely used smoking biomarkers. Among these biomarkers, urinary cotinine has been one of representative specific for tobacco smoke exposure (3)(4)(5) with regard to active or passive smoking. The methods frequently quantification gas chromatography (6) HPLC (7), coupled not mass spectroscopy (8)(9). These methods, however, difficult use in large-scale epidemiological studies because they require specialized laboratories. In 1973, Langone et al. (10) proposed assessment by RIA, but RIAs also This last technique was then extended ELISA (11) fluorescence polarization immunoassay (12). Recently, an enzyme (EIA) that is easier perform (13) developed measure concentrations between 100 2000 μg/L, a range exceeds observed We thus propose adaptation automation this EIA assess <100 μg/L detect After analytical validation, applied subjects exposed environmental (ETS). All chemicals, …