(−)-Epigallocatechin-3-gallate inhibits matrix metalloproteinases in oral ulcers

摘要: The overexpression of matrix metalloproteinases (MMPs) results in excessive extracellular degradation and oral ulcer healing delay. This study investigated the inhibitory effect (−)-epigallocatechin-3-gallate (EGCG) on MMPs, possible molecular mechanism its implications for ulcers. MMP-1 MMP-9 inhibition was detected using fluorometric colorimetric assays after incubating EGCG (at concentrations 25, 50, 100 200 μM) with along their specific substrates. recombinant human (rh) interleukin-1β (rhIL-1β)-induced production by macrophages measured enzyme-linked immunosorbent (ELISAs). 75% acetic acid used to prepare experimental animal model. expression observed an immunohistochemical assay western blot analysis. activity activator protein (AP-1) were determined real-time PCR luciferase assay, respectively. revealed that, vitro, inhibited MMPs rhIL-1β-induced MMP a concentration-dependent manner. constants (Ki) 9.56 ± 1.67 26.36 1.85 μM, In vivo, means positive integrated optic density (IOD) expressions control group significantly higher than those large dose group. showed that c-fos c-jun expression, as well AP-1 transcriptional activity, dose-dependent Thus, has capability accelerate ulcers inhibiting MMP-9. may involve deactivation AP-1.