Regulation of matrix metalloproteinase secretion by green tea catechins in a three-dimensional co-culture model of macrophages and gingival fibroblasts.

摘要: Abstract Objectives Elevated levels of matrix metalloproteinases (MMPs) have been associated with the active phases tissue and bone destruction in periodontitis, an inflammatory disease characterized by a significant breakdown tooth support. In present study, we used three-dimensional (3D) co-culture model macrophages gingival fibroblasts to investigate ability green tea extract its major constituent epigallocatechin-3-gallate (EGCG) regulate secretion MMP-3, -8, -9. Methods The 3D was composed embedded type I collagen overlaid macrophages. Two arbitrary ratios were tested. ratio 1 macrophage 10 mimic slightly inflamed periodontal site while fibroblast severely site. pre-treated for 2 h either or EGCG. It then stimulated Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS). also first LPS incubated concentrations secreted -9 quantified enzyme-linked immunoassays. Results When A. LPS, 10:1 highest MMP-3 and, lesser extent, MMP-8, than 1:10 ratio. Non-cytotoxic EGCG reduced basal all three MMPs. A 2-h treatment prior stimulation resulted dose-dependent decrease MMP secretion, MMP-9 showing most decrease. observed when added following LPS. Conclusions Our results suggested that catechins, more specifically EGCG, offer promising prospects development novel adjunctive periodontitis because their MMPs, which are important tissue-destructive enzymes produced mucosal immune cells.