17β-Estradiol up-regulates miR-155 expression and reduces TP53INP1 expression in MCF-7 breast cancer cells

摘要: In estrogen responsive breast cancer cells, estradiol (E2) is a key regulator of cell proliferation and survival. MiR-155 has emerged as an "oncomiR", which the most significantly up-regulated miRNA in cancer. Moreover, miR-155 higher ERα (+) tumors than (-), but no one examined whether E2 regulates expression MCF-7 cells. this study, aim was to explore involved regulated genes. We evaluated human cells by real-time PCR, finding out overexpressed compared with MDA-MB-231 Treatment increased expression, promoting decreasing apoptosis, similarly, transfection miR-155m gave similar results. contrast, inhibited inhibitors reduced promoted apoptosis TP53INP1 targets miR-155. negatively mRNA protein TP53INP1, cleaved-caspase-3, -8, -9, p21, whereas p21 level. These results demonstrated that development progression possibly through increasing miR-155, contributes down-regulating TP53INP1.