A micro-autoanalytic procedure developed for the determination of ketone bodies, gluconeogenic amino acids, pyruvate, lactate, and glucose in metabolic studies
作者： P.T. Ozand , R.L. Hawkins , R.M. Collins , J.T. Tildon , M. Cornblath
DOI: 10.1016/0006-2944(75)90034-4
关键词:
摘要: Abstract A micro-autoanalytic procedure for the enzymic, fluorometric determinations of acetoacetate, β-hydroxybutyrate, pyruvate, lactate, alanine, glucose, glutamate, and glutamine has been described. As many as 60 to 80 duplicate samples can be analyzed simultaneously either two or three compounds in 8 hr. The sensitivity range detection permit analyses all eight using only 50 μl blood. reproducibility, efficiency, versatility this autoanalytic system will warrant its wide application metabolic studies.
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