Chromatin Immunoprecipitation Indirect Peaks Highlight Long-Range Interactions of Insulator Proteins and Pol II Pausing
作者： Jun Liang , Laurent Lacroix , Adrien Gamot , Suresh Cuddapah , Sophie Queille
DOI: 10.1016/J.MOLCEL.2013.12.029
关键词:
摘要: Eukaryotic chromosomes are partitioned into topologically associating domains (TADs) that demarcated by distinct insulator-binding proteins (IBPs) in Drosophila. Whether IBPs regulate specific long-range contacts and how this may impact gene expression remains unclear. Here we identify “indirect peaks” of multiple represent their distant sites interactions through contacts. Indirect peaks depend on protein-protein among common cofactors, including CP190, as confirmed high-resolution analyses Mutant unable to interact with CP190 impair well the hundreds genes specifically flanked indirect peaks. Regulation strongly correlates RNAPII pausing, highlighting key transcriptional stage trap insulator-based interactions. Our data illustrate decipher regulatory networks
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