Effective management of microbial contamination in cultured skin substitutes after grafting to athymic mice.

摘要: Cultured skin substitutes have become therapeutic alternatives for treatment of acute and chronic wounds, but all models these are avascular susceptible to microbial destruction during vascularization. To develop a practical management protocol increased survival substitutes, experimental wounds were contaminated with Pseudomonas aeruginosa treated formulation noncytotoxic antimicrobial agents (polymyxin B, neomycin, ciprofloxacin, mupirocin, amphotericin B) in nutrient medium (vehicle). consisting human keratinocytes fibroblasts attached collagen-glycosaminoglycan sponges grafted 2 x cm full-thickness on athymic mice that the strain SBI-N P. at 1 10(4), 10(5), 10(6) organisms/wound. Experimental irrigated ml/day topical solution 10 days, controls received vehicle only. Two, three, four weeks after grafting, traced swabbed culture areas measured planimetry. At 4 weeks, biopsy samples scored histochemically immunoreactivity HLA antigens. Data analysis by chi-square, variance, Tukey's test shows antimicrobials is associated an area healed positive detection antigens, negative cultures aeruginosa. These results show contamination cultured may be managed effectively graft However, this not currently approved use investigational Effective suggests clinical efficacy tissue analogs local application agents.