Isolation and Quantification of Metabolite Levels in Murine Tumor Interstitial Fluid by LC/MS.

摘要: Cancer is a disease characterized by altered metabolism, and there has been renewed interest in understanding the metabolism of tumors. Even though nutrient availability critical determinant tumor little systematic study nutrients directly available to cancer cells microenvironment. Previous work characterizing metabolites present interstitial fluid restricted measurement small number such as glucose lactate limited samples. Here we adapt centrifugation-based method isolation readily applicable sample types mass spectrometry-based for absolute quantitation many In this method, (TIF) analyzed liquid chromatography-mass spectrometry (LC/MS) using both isotope dilution external standard calibration derive concentrations targeted fluid. The use allows accurate metabolites, other methods are inadequate determining biological fluids due matrix effects that alter apparent concentration depending on composition which they contained. This therefore can be applied measure from diverse types, well most fluids, allowing characterization levels microenvironment solid