Flow cytometric analysis of procalcitonin expression in human monocytes and granulocytes.
作者： Attila Balog , Imre Ocsovszki , Yvette Mándi
DOI: 10.1016/S0165-2478(02)00158-X
关键词:
摘要: Abstract Procalcitonin (PCT), a precursor of calcitonin is useful indicator severe systemic infection and sepsis. For better understanding the pathophysiological background PCT induction, study was made intracellular expression in various human white blood cell populations—i.e. monocytes polymorphonuclear granulocytes (PMNs)—and role TNF-α stimulation their production. The investigated by flow cytometric analysis with staining antibodies to components (CT) katacalcin (KC). Both peripheral expressed PCT, increased amounts were demonstrated after Staphylococcus aureus as inducer. S. induced production inhibited anti-TNF-α monoclonal antibodies. monocytic line U937 considerable but failed induce an increase expression. determination cytometry promising sensitive method for further investigation effects cytokines cytokine-inducing agents synthesis granulocytes.
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