Reduced lesions in chickens with Clostridium perfringens-induced necrotic enteritis by Lactobacillus fermentum 1.2029

摘要: Previous studies have demonstrated that Lactobacillus has anti-inflammatory properties, but the protective functions of and mechanisms inhibition necrotic enteritis (NE) in intestines chickens not been fully clarified. In present study, we selected a probiotic strain, fermentum 1.2029, which good adhesive ability high survival rate low pH bile salts. The objective this study was to examine properties L. 1.2029 against NE chickens. Two hundred forty 1-d-old male Arbor Acres broilers were blocked into 3 experimental groups as follows: (I) nonchallenge control group, (II) Clostridium perfringens challenge (III) C. + group. (1.0 mL/d, 10(8) cfu/mL) orally administered daily group III during course experiment, all uninfected inoculated accordingly with same volume PBS. (0.5 mL on d 1 1.0 14 21, II. At 28 d, scoring gross lesions performed. Ileal segments approximately 2 cm from 24 each collected fixed 4% (wt/vol) neutral-buffered formalin solution for histological scoring. mucosa samples also mRNA analysis by real-time PCR. results showed reduced severity Histological scores revealed inflammation damage Changes cytokines Toll-like receptors (TLR) determined, found increase interleukin-10 levels reduce interferon-γ TLR2 NE-infected able regulate intestinal mucosal immune response ameliorate changing expression TLR.