Characterization of circulating osteoblast lineage cells in humans.

摘要: Abstract We recently identified circulating osteoblastic cells using antibodies to osteocalcin (OCN) or alkaline phosphatase (AP). now provide a more detailed characterization of these cells. Specifically, we demonstrate that 46% OCN positive (OCNpos) express AP, and 37% also the hematopoietic/endothelial marker CD34. Using two different anti-OCN forward/side light scatter characteristics by flow cytometry, find OCNpos consist distinct populations: one population exhibits low scatter, consistent with small cell phenotype granularity, second has higher (larger granular cell). The smaller, granularity co-expresses CD34, whereas larger, are CD34 negative. samples from 26 male subjects aged 28 68 years, concentration increases as function age (R = 0.59, P = 0.002). By contrast, CD34pos tend decrease (R = − 0.31, P = 0.18); consequence, ratio OCNpos:CD34pos increase significantly (R = 0.54, P = 0.022). These findings suggest significant overlap between expressing those Further studies needed define precise role not only in bone remodeling but rather potentially response vascular injury.