Humoral and cellular immune responses to airway immunization of mice with human papillomavirus type 16 virus-like particles and mucosal adjuvants.

摘要: Cervical cancer results from cervical infection by human papillomaviruses (HPV), especially HPV16. Intramuscular administrations of HPV16 virus-like particle (VLP) vaccines have been shown to induce strong neutralizing antibody responses and protect women against genital associated lesions. However, an alternative route administration that avoids parenteral injection might facilitate vaccine implementation, particularly in developing countries which account for the majority worldwide cases cancer. In addition, inducing mucosal immunity could partially overcome substantial variation antibodies at cervix seen ovulating women. Aerosol vaccination with VLPs was previously be immunogenic mice Here, we examine whether exposure other respiratory viral antigens may interfere VLP-specific humoral response two known adjuvants, CpG oligodeoxynucleotides a natural non-toxic Escherichia coli heat-labile enterotoxin (HLT), can enhance immunogenicity airway immunization (nasal or aerosol-like) VLPs. Our data show HLT significantly improve anti-VLP serum secretions, as well proliferative IFN-γ production CD8 T cells, recent influenza surface diminish mucosal, but not systemic,