作者: R.L. Tarleton , R.E. Kuhn
DOI: 10.1016/0022-1759(83)90349-6
关键词:
摘要: Abstract A solid phase radioimmunoassay (RIA) to measure parasite-specific antibody responses has been developed. The assay utilizes 3 H-labeled avidin as the indicator molecule and represents a marked improvement in detection of specific antibodies Trypanosoma cruzi over previously used RIA. Avidin is easily labeled high efficiency (3.6 × 10 5 cpm/ μ g) under very mild conditionds using N-succinimidyl-[2,3- H]propionate, avidin-biotin binding coupled with increased safety longer half-life reagent give this method advantage currently 125 I-labeled Ab RIAs.