Measurement of parasite-specific antibody responses using a tritiated avidin-solid phase radioimmunoassay

作者: R.L. Tarleton , R.E. Kuhn

DOI: 10.1016/0022-1759(83)90349-6

关键词:

摘要: Abstract A solid phase radioimmunoassay (RIA) to measure parasite-specific antibody responses has been developed. The assay utilizes 3 H-labeled avidin as the indicator molecule and represents a marked improvement in detection of specific antibodies Trypanosoma cruzi over previously used RIA. Avidin is easily labeled high efficiency (3.6 × 10 5 cpm/ μ g) under very mild conditionds using N-succinimidyl-[2,3- H]propionate, avidin-biotin binding coupled with increased safety longer half-life reagent give this method advantage currently 125 I-labeled Ab RIAs.

参考文章(11)
D W Taylor, C B Evans, R Asofsky, K J Kim, Radioimmunoassay for detecting antibodies against murine malarial parasite antigens: monoclonal antibodies recognizing Plasmodium yoelii antigens. Journal of Immunology. ,vol. 125, pp. 2565- 2569 ,(1980)
B O Brooks, D L Wassom, R H Cypess, Kinetics of immunoglobulin M and G responses of nude and normal mice to Trypanosoma musculi. Infection and Immunity. ,vol. 36, pp. 667- 671 ,(1982) , 10.1128/IAI.36.2.667-671.1982
Robert G. Hamilton, Rabia Hussain, Eric A. Ottesen, N. Franklin Adkinson, The quantitation of parasite-specific human IgG and IgE in sera: evaluation of solid-phase RIA and ELISA methodology. Journal of Immunological Methods. ,vol. 44, pp. 101- 114 ,(1981) , 10.1016/0022-1759(81)90111-3
Ronald L. Wilder, Catherine C. Yuen, Bondada Subbarao, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage, Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays. Journal of Immunological Methods. ,vol. 28, pp. 255- 266 ,(1979) , 10.1016/0022-1759(79)90192-3
Susan Jackson, John A. Sogn, Thomas J. Kindt, Microdetermination of rabbit immunoglobulin allotypes by ELISA using specific antibodies conjugated with peroxidase or with biotin Journal of Immunological Methods. ,vol. 48, pp. 299- 309 ,(1982) , 10.1016/0022-1759(82)90331-3
Raymond E. Kuhn, John E. Murnane, Trypanosoma cruzi: Immune destruction of parasitized mouse fibroblasts in vitro Experimental Parasitology. ,vol. 41, pp. 66- 73 ,(1977) , 10.1016/0014-4894(77)90130-8
I. Doxiadis, M. Eulitz, S. Sladoljev, S. Thierfelder, U. Kummer, E. Thiel, Tritium radiolabeling of antibodies to high specific activity with N-succinimidyl [2,3-3H] propionate: use in detecting monoclonal antibodies. Journal of Immunological Methods. ,vol. 42, pp. 367- 374 ,(1981) , 10.1016/0022-1759(81)90165-4