Simultaneous emergence and rapid spread of three OXA-23 producing Acinetobacter baumannii ST208 strains in intensive care units confirmed by whole genome sequencing.

摘要: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a common nosocomial bacterial pathogen with limited treatment options. CRAB outbreaks are disastrous for critically ill patients. This study investigated carbapenemase-produced A. in tertiary hospital. Although multiple were suggested by pulse-field gel electrophoresis, the genetic lineages and evolution between these isolates not clear. To investigate genomic epidemiology of to reveal possible transmission routes, whole genome sequences (WGS) compared analyzed. From WGS data, thirty had same sequence type (ST208); acquired resistance genes chromosome resistant detected responsible multidrug resistance. A phylogenetic tree single-nucleotide polymorphisms (SNPs) earliest index isolate found that three emerged disseminated simultaneously. Of these, <10 SNPs within cluster, whereas at least 600 clusters. The probable routes generated combined distance patient epidemiological data. In conclusion, was convenient accurate monitoring method epidemiologic investigation outbreaks, surveillance multidrug-resistant pathogens would be powerful warning system prevention outbreaks.