Transcriptional modulation of micro-RNA in human cells differing in radiation sensitivity.
作者： M. Ahmad Chaudhry , Bridget Kreger , Romaica A. Omaruddin
DOI: 10.3109/09553001003734568
关键词:
摘要: Purpose: The molecular basis of gene regulation in cells exposed to ionising radiation is not fully understood. Gene occurs at the transcriptional and post-transcriptional levels. Recent studies have suggested that micro-RNA (miRNA) plays a significant role regulation. miRNA are recently identified class RNA molecules 18–24 nucleotides length been shown negatively regulate stability or translation target messenger RNA. We hypothesised induced stress response controlled part by difference tumour protein 53 (p53) status corresponds with altered expression responses radiation.Materials methods: To test this hypothesis, we investigated relative several quantitative real-time polymerase chain reaction (QPCR) human cell lines TK6 WTK1 differ p53 radiosensitivity after exposure high low doses X-radiation.R...
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