Amplification of c-erbB2 oncogene

摘要: BACKGROUND Overexpression of the epidermal growth factor type II receptor HER-2/neu has been associated with resistance to chemotherapy and poor survival in several human tumors. In current study, authors have determined frequency clinical significance gene amplification uterine serous papillary endometrial carcinoma (USPC), a highly aggressive variant carcinoma. METHODS Fluorescence situ hybridization (FISH) assay was used analyze paraffin blocks from 30 women harboring Stage IA–IV USPC treated at University Arkansas for Medical Sciences (Little Rock, AR) 1997 2004. Chromosome 17 polysomy status by FISH also assessed all specimens. patient relation analyzed using Kaplan–Meier curves conjunction log-rank test. RESULTS Amplification observed 14 (47%) cases. Heterogeneity noted 4 cases within same tumor samples pockets amplified cells amidst nonamplified cells. Patients tumors had significantly shorter time diagnosis disease-related death when compared FISH-negative patients (P = 0.0008). African-American (AA) were found poorer prognosis Caucasian (C) 0.01) harbor higher levels 0.02). CONCLUSIONS HER-2/neu be an important prognostic indicator outcome that occurs more frequently AA C patients. Determination may guide management implications implementation novel treatment strategies. Cancer 2005. © 2005 American Society.