Next-Generation Sequencing.

摘要: Endometrial cancers are the most frequently diagnosed gynecological malignancy and were expected to be seventh leading cause of cancer death among American women in 2015. The majority endometrial serous or endometrioid histology. Most human tumors, including driven by acquisition pathogenic mutations genes. Thus, identification somatic within tumor genomes is an entry point toward gene discovery. However, efforts pinpoint have, until recently, relied on high-throughput sequencing single genes families using Sanger sequencing. Although this approach has been fruitful, cost throughput generally prohibits systematic ~22,000 that make up exome. recent development next-generation technologies changed paradigm providing capability rapidly sequence exomes, transcriptomes, at relatively low cost. Remarkably, application technology catalog mutational landscapes revealed, for first time, can classified into four distinct molecular subgroups. In chapter, we overview characteristic genomic features each subgroup discuss known putative have emerged from carcinomas.